小鼠蛋白酶体亚基lmp10（Psmb10）基因的DNA序列、染色体定位及组织表达

DNA sequence, chromosomal localization, and tissue expression of the mouse proteasome subunit lmp10 (Psmb10) gene.

作者信息

Cruz M, Elenich L A, Smolarek T A, Menon A G, Monaco J J

机构信息

Department of Molecular Genetics, Howard Hughes Medical Institute, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, Ohio, 45267-0524, USA.

出版信息

Genomics. 1997 Nov 1;45(3):618-22. doi: 10.1006/geno.1997.4977.

DOI:10.1006/geno.1997.4977

PMID:9367687

Abstract

Proteasomes are nonlysosomal multicatalytic proteases involved in antigen processing. Three of the 10 mammalian proteasome beta subunits (LMP2, LMP7, and LMP10) are induced by IFN-gamma. Two of these (LMP2 and LMP7) are encoded in the major histocompatibility complex of both human (chromosome 6) and mouse (chromosome 17). However, the human homologue of Lmp10, MECL1, is found on chromosome 16. Here we show that in mice, Lmp10 is a single-copy gene localized to chromosome 8, in a region of conserved synteny with human chromosome 16. Sequencing of a 129/SvJ strain genomic clone revealed that the gene has eight exons spanning 2.3 kb. Characterization of a full-length mouse cDNA clone indicates that Lmp10 encodes a protein of 273 amino acids with a calculated molecular weight of 29 kDa and an isoelectric point of 6.86. Northern analysis of Lmp2, Lmp7, and Lmp10 showed expression in heart, liver, thymus, lung, and spleen, but not in brain, kidney, skeletal muscle, or testis.

摘要

蛋白酶体是参与抗原加工的非溶酶体多催化蛋白酶。10种哺乳动物蛋白酶体β亚基中的3种（LMP2、LMP7和LMP10）由γ干扰素诱导产生。其中两种（LMP2和LMP7）在人类（6号染色体）和小鼠（17号染色体）的主要组织相容性复合体中编码。然而，Lmp10的人类同源物MECL1位于16号染色体上。在此我们表明，在小鼠中，Lmp10是一个单拷贝基因，定位于8号染色体，位于与人类16号染色体保守同线性的区域。对129/SvJ品系基因组克隆进行测序显示，该基因有8个外显子，跨度为2.3 kb。对一个全长小鼠cDNA克隆的鉴定表明，Lmp10编码一种273个氨基酸的蛋白质，计算分子量为29 kDa，等电点为6.86。对Lmp2、Lmp7和Lmp10的Northern分析表明，它们在心脏、肝脏、胸腺、肺和脾脏中表达，但在脑、肾、骨骼肌或睾丸中不表达。