Ishizuka S, Nagashima Y, Numata M, Yano T, Hagiwara K, Ozasa H, Sone M, Nihei H, Horikawa S
Fourth Department of Internal Medicine, Tokyo Women's Medical College, Japan.
Biochem Biophys Res Commun. 1997 Nov 7;240(1):93-8. doi: 10.1006/bbrc.1997.7573.
Intramuscular injection of hypertonic glycerol solution to rats results in acute renal injury. In this model, the proximal tubules are characteristically damaged. After glycerol injection renal glutathione (GSH) levels drastically decreased. On the other hand, stress protein heme oxygenase-1 (HO-1) was induced. When N-acetyl cysteine was administered to rats before 1 h glycerol injection, renal function was obviously improved. In this condition, the renal GSH content were sustained in the normal levels and HO-1 protein levels were decreased compared with those of glycerol-treated rats. Induction of HO-1 was accompanied by reduced renal GSH content. In addition, to investigate whether the location of HO-1 protein induced by glycerol injection is restricted to injured region or not in the kidney, we determined the localization of HO-1 protein using immunohistochemical staining. HO-1 protein was identified in the epithelia of the distal tubules, Henle's loop and collecting ducts, but not in the injured proximal tubules.
给大鼠肌肉注射高渗甘油溶液会导致急性肾损伤。在这个模型中，近端肾小管会受到典型损伤。注射甘油后，肾脏谷胱甘肽（GSH）水平急剧下降。另一方面，应激蛋白血红素加氧酶-1（HO-1）被诱导产生。在注射甘油前1小时给大鼠施用N-乙酰半胱氨酸，肾功能明显改善。在此条件下，肾脏GSH含量维持在正常水平，与甘油处理的大鼠相比，HO-1蛋白水平降低。HO-1的诱导伴随着肾脏GSH含量的减少。此外，为了研究注射甘油诱导的HO-1蛋白在肾脏中的定位是否仅限于损伤区域，我们使用免疫组织化学染色确定了HO-1蛋白的定位。HO-1蛋白在远端小管、髓袢和集合管的上皮细胞中被鉴定出来，但在受损的近端小管中未被发现。
