Sun J S, Tsuang Y H, Liao C J, Liu H C, Hang Y S, Lin F H
Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, Republic of China.
J Biomed Mater Res. 1997 Dec 5;37(3):324-34. doi: 10.1002/(sici)1097-4636(19971205)37:3<324::aid-jbm3>3.0.co;2-n.
With advances in ceramics technology, calcium phosphate bioceramics have been applied as bone substitutes for several decades. The focus of this work is to elucidate the biocompatibility of the particulates of various calcium phosphate cytotoxicities. Four different kinds of calcium phosphate powders, including beta-tricalcium phosphate (beta-TCP), hydroxyapatite (HA), beta-dicalcium pyrophosphate (beta-DCP), and sintered beta-dicalcium pyrophosphate (SDCP), were tested by osteoblast cell culture. The results were analyzed by cell count, concentration of transforming growth factor-beta 1 (TGF-beta 1), alkaline phosphatase (ALP), and prostaglandin E2 (PGE2) in culture media. The changes were most significant when osteoblasts were cultured with beta-TCP and HA bioceramics. The changes in cell population of the beta-TCP and HA were quite low in the first 3 days, then increased gradually toward the seventh day. The changes in TGF-beta 1 concentration in culture medium inversely related to the changes in cell population. The ALP titer in the culture media of the beta-TCP and HA were quite high in the first 3 days, then decreased rapidly between the third and seventh days. The concentrations of PGE2 in the culture media tested were quite high on the first day, decreased rapidly to the third day, and then gradually until the seventh day. The changes in the beta-DCP and SDCP were quite similar to those of HA and beta-TCP but much less significant. We conclude that HA and beta-TCP have an inhibitory effect on the growth of osteoblasts. The inhibitins effects of the HA and beta-TCP powders on the osteoblast cell cultures possibly are mediated by the increased synthesis of PGE2.
随着陶瓷技术的进步，磷酸钙生物陶瓷作为骨替代物已应用了几十年。这项工作的重点是阐明各种磷酸钙细胞毒性颗粒的生物相容性。通过成骨细胞培养测试了四种不同的磷酸钙粉末，包括β-磷酸三钙（β-TCP）、羟基磷灰石（HA）、β-焦磷酸二钙（β-DCP）和烧结β-焦磷酸二钙（SDCP）。通过培养基中细胞计数、转化生长因子-β1（TGF-β1）、碱性磷酸酶（ALP）和前列腺素E2（PGE2）的浓度对结果进行分析。当成骨细胞与β-TCP和HA生物陶瓷一起培养时，变化最为显著。β-TCP和HA的细胞数量在前3天变化很小，然后在第7天逐渐增加。培养基中TGF-β1浓度的变化与细胞数量的变化呈负相关。β-TCP和HA培养基中的ALP滴度在前3天相当高，然后在第3天至第7天迅速下降。测试的培养基中PGE2的浓度在第一天相当高，在第三天迅速下降，然后在第7天逐渐下降。β-DCP和SDCP的变化与HA和β-TCP的变化非常相似，但不太显著。我们得出结论，HA和β-TCP对成骨细胞的生长有抑制作用。HA和β-TCP粉末对成骨细胞培养的抑制作用可能是由PGE2合成增加介导的。
