Sung H W, Hsu H L, Hsu C S
Department of Chemical Engineering, National Central University, Chung-Li, Taiwan, Republic of China.
J Biomed Mater Res. 1997 Dec 5;37(3):376-83. doi: 10.1002/(sici)1097-4636(19971205)37:3<376::aid-jbm8>3.0.co;2-i.
Due to the nature of bioprostheses, which are mainly biological tissues that cannot be sterilized with heat or irradiation, the sterilization method by choice is generally liquid chemicals. It is known that a number of liquid chemicals can have rapid germicidal effect and can be used to sterilize bioprostheses. The study was to evaluate the effects of various chemical sterilization methods on the crosslinking and enzymatic degradation characteristics of an epoxy-fixed biological tissue. The chemical sterilants employed were: a 70% ethanol solution (EtOH), a 2% epoxy compound + 20% ethanol solution (EX-810), a 2% propylene oxide + 20% ethanol solution (PO), and a 0.625% glutaraldehyde + 20% ethanol + 0.2% polysorbate solution (GA). Both masking and crosslinking of the free amino groups within the epoxy-fixed tissue were observed subsequent to sterilization with GA or EX-810. This improved the resistance of the GA or EX-810 sterilized tissues against collagenase degradation as compared to its nonsterilized counterpart. However, subsequent to sterilization with PO, only masking of the free amino groups within the epoxy-fixed tissue was noted. The inhibition of the collagenase degradation by masking of the free amino groups was traded off by the more random molecular packing of the PO sterilized sample due to the introduction of the side branches. Sterilization of the epoxy-fixed tissue with EtOH may increase its denaturation temperature and tensile strength, while neither masking nor crosslinking of free amino groups within the tissue took place. The resistance to degradation of the EtOH sterilized tissue, however, did not improve as compared to its nonsterilized counterpart.
由于生物假体的性质，其主要是不能用加热或辐照进行灭菌的生物组织，通常选择的灭菌方法是液体化学药剂。已知多种液体化学药剂具有快速杀菌作用，可用于生物假体的灭菌。本研究旨在评估各种化学灭菌方法对环氧固定生物组织的交联和酶降解特性的影响。所使用的化学消毒剂有：70%乙醇溶液（EtOH）、2%环氧化合物+20%乙醇溶液（EX-810）、2%环氧丙烷+20%乙醇溶液（PO）以及0.625%戊二醛+20%乙醇+0.2%聚山梨酯溶液（GA）。在用GA或EX-810灭菌后，观察到环氧固定组织内游离氨基的掩蔽和交联现象。与未灭菌的对应组织相比，这提高了GA或EX-810灭菌组织对胶原酶降解的抵抗力。然而，在用PO灭菌后，仅注意到环氧固定组织内游离氨基的掩蔽现象。由于侧链的引入，PO灭菌样品分子排列更无序，通过掩蔽游离氨基对胶原酶降解的抑制作用被抵消。用EtOH对环氧固定组织进行灭菌可能会提高其变性温度和拉伸强度，而组织内游离氨基既未发生掩蔽也未发生交联。然而，与未灭菌的对应组织相比，EtOH灭菌组织的抗降解能力并未提高。
