Vervoordeldonk S F, van den Berg H, von dem Borne A E, van Leeuwen E F, Slaper-Cortenbach I C
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, The Netherlands.
J Hematother. 1997 Oct;6(5):495-500. doi: 10.1089/scd.1.1997.6.495.
In our laboratory, a two-step procedure is used for purging precursor B ALL from autologous bone marrow grafts of children in second bone marrow remission. An immunorosette depletion method with CD19 and CD22 MAbs is followed by one cycle of complement-mediated cell lysis with CD9 and CD10 MAbs. The aim of the present study was to determine if the efficacy of this procedure could be further enhanced by including CD20 and CD72 MAbs in the current protocol. Leukemia-contaminated remission bone marrow was simulated by mixing cell line cells and normal bone marrow cells. The efficacy of purging of malignant cells was determined by culturing the cells in a limiting dilution assay. The effect of including CD20 and CD72 in the immunorosette depletion was limited. In contrast, when these MAbs were added during complement-mediated cell lysis, a significant increase in depletion of tumor cells was observed. This was true when complement lysis was carried out alone (0.4 versus 3.0 log depletion for Ros cells) and when it was preceded by immunorosette depletion (2.7 versus 4.1 log depletion for Ros cells). The loss of hematopoietic progenitor cells was not greater than with the current purging protocol.
在我们实验室中，采用两步法从处于第二次骨髓缓解期的儿童自体骨髓移植物中清除前体B淋巴细胞白血病细胞。先用抗CD19和抗CD22单克隆抗体进行免疫玫瑰花环去除法，随后用抗CD9和抗CD10单克隆抗体进行一轮补体介导的细胞裂解。本研究的目的是确定在当前方案中加入抗CD20和抗CD72单克隆抗体是否能进一步提高该方法的疗效。通过将细胞系细胞与正常骨髓细胞混合来模拟被白血病污染的缓解期骨髓。通过有限稀释分析培养细胞来确定清除恶性细胞的疗效。在免疫玫瑰花环去除法中加入抗CD20和抗CD72的效果有限。相比之下，当在补体介导的细胞裂解过程中加入这些单克隆抗体时，观察到肿瘤细胞清除率显著提高。单独进行补体裂解时（玫瑰红结细胞的清除率为0.4对3.0对数）以及在免疫玫瑰花环去除法之前进行补体裂解时（玫瑰红结细胞的清除率为2.7对4.1对数）都是如此。造血祖细胞的损失并不比当前的清除方案更大。
