Soldatovic D, Vujanovic D, Matovic V, Plamenac Z
Department of Toxicol. Chemistry, Faculty of Pharmacy, University of Belgrade, Yugoslavia.
Magnes Res. 1997 Jun;10(2):127-33.
Our previous experiments showed that excessive oral Mg intake has beneficial effects in chronic Pb poisoning, inducing decrease of Pb body burden and its increased elimination via urine. The aim of this work was to investigate and to compare the effect of Mg on urinary Pb elimination with the effect of calcium-disodium edetate (CaNa2EDTA)--chelating agent currently used in therapy of chronic Pb intoxication. Besides, under the same experimental conditions, biochemical parameters protoporphyrin IX (ppIX), zinc protoporphyrin (Znpp) and delta-aminolevulinic acid (ALA) were determined. Experiments were carried out on rabbits previously intoxicated for 4 weeks with 20 mg Pb/kg b.w. per day. After the period of intoxication, one group of animals was given per os, for 28 days, 40 mg Mg/kg, the other one was i.v. treated for 7 days with 15 mg CaNa2EDTA/kg (therapeutic doses), while the third one (rabbits without therapy) served as a control. During the period of detoxication, lead was determined in blood and urine samples, and ppIX and Znpp were determined in blood and ALA in urine. Results suggest that oral treatment with magnesium, although inducing later Pb elimination than EDTA, has even better effect on investigated biochemical parameters than chelating therapy.
我们之前的实验表明，过量口服镁对慢性铅中毒有有益作用，可导致体内铅负荷降低，并通过尿液增加铅的排泄。这项工作的目的是研究并比较镁对尿铅排泄的影响与依地酸钙钠（CaNa2EDTA）——目前用于治疗慢性铅中毒的螯合剂——的影响。此外，在相同的实验条件下，测定了生化参数原卟啉IX（ppIX）、锌原卟啉（Znpp）和δ-氨基乙酰丙酸（ALA）。实验用先前每天以20毫克铅/千克体重中毒4周的兔子进行。中毒期过后，一组动物口服40毫克镁/千克，持续28天，另一组静脉注射15毫克依地酸钙钠/千克，持续7天（治疗剂量），而第三组（未接受治疗的兔子）作为对照。在解毒期间，测定血液和尿液样本中的铅，测定血液中的ppIX和Znpp以及尿液中的ALA。结果表明，口服镁治疗虽然比依地酸二钠钙导致铅排出的时间晚，但对所研究的生化参数的影响比螯合疗法更好。
