Bates E E, Ravel O, Dieu M C, Ho S, Guret C, Bridon J M, Ait-Yahia S, Brière F, Caux C, Banchereau J, Lebecque S
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Eur J Immunol. 1997 Oct;27(10):2471-7. doi: 10.1002/eji.1830271002.
Using a cDNA subtraction technique, a novel member of the ubiquitin family was isolated from human dendritic cells. This gene encodes a diubiquitin protein containing tandem head to tail ubiquitin-like domains, with the conservation of key functional residues. Expression of this 777-bp mRNA was restricted to dendritic cells and B cells, with strong expression in mature B cells. Southern blot analysis indicated that a single copy of this gene is present. In situ hybridization on tonsillar tissue showed expression in epithelial cells and isolated cells within the germinal center. With respect to an expressed-sequence tag (EST) this cDNA could be localized to the major histocompatibility complex class I region of chromosome 6. Comparative analysis and the expression pattern of this gene suggests a function in antigen processing and presentation.
利用cDNA消减技术，从人树突状细胞中分离出泛素家族的一个新成员。该基因编码一种含有串联头尾相连泛素样结构域的双泛素蛋白，关键功能残基保守。这种777碱基对的mRNA的表达仅限于树突状细胞和B细胞，在成熟B细胞中表达强烈。Southern印迹分析表明该基因以单拷贝形式存在。扁桃体组织的原位杂交显示生发中心的上皮细胞和分离细胞中有表达。关于一个表达序列标签（EST），该cDNA可定位于6号染色体的主要组织相容性复合体I类区域。该基因的比较分析和表达模式表明其在抗原加工和呈递中发挥作用。
