病毒启动子在CEM-C7和Jurkat人T淋巴细胞系中的表达。

Viral promoter expression in CEM-C7 and Jurkat human T-lymphoid cell lines.

作者信息

Sutherland L C, Williams G T

机构信息

Department of Biological Sciences, Keele University, Staffordshire, UK.

出版信息

J Immunol Methods. 1997 Sep 24;207(2):179-83. doi: 10.1016/s0022-1759(97)00118-x.

DOI:10.1016/s0022-1759(97)00118-x

PMID:9368644

Abstract

The strength of various viral promoters was examined in human T lymphoblastoid cell lines. CMV, RSV and SV40 promoter based-luciferase constructs were transiently transfected into CEM-C7 and Jurkat cells in order to compare promoter strength in each cell type. It was found that the CMV promoter was 10-fold stronger than the RSV promoter in Jurkat cells, but equivalent to the RSV promoter in CEM-C7 cells. Both the CMV and RSV promoters were significantly stronger than the SV40 promoter in Jurkat and CEM-C7 cells. In summary, promoter strengths are cell line specific (Jurkat: CMV > RSV >> SV40; CEM-C7: RSV = CMV >> SV40) and care must be exercised in choosing promoters intended to provide overexpression of chimeric genes.

摘要

在人T淋巴母细胞系中检测了各种病毒启动子的强度。基于巨细胞病毒（CMV）、劳斯肉瘤病毒（RSV）和猴空泡病毒40（SV40）启动子的荧光素酶构建体被瞬时转染到CEM-C7和Jurkat细胞中，以便比较每种细胞类型中的启动子强度。发现在Jurkat细胞中，CMV启动子比RSV启动子强10倍，但在CEM-C7细胞中与RSV启动子相当。在Jurkat和CEM-C7细胞中，CMV和RSV启动子均明显强于SV40启动子。总之，启动子强度具有细胞系特异性（Jurkat：CMV > RSV >> SV40；CEM-C7：RSV = CMV >> SV40），在选择旨在提供嵌合基因过表达的启动子时必须谨慎。