Fujikawa-Yamamoto K, Zong Z P, Murakami M, Odashima S, Ikeda T, Yoshitake Y
Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
Cell Struct Funct. 1997 Aug;22(4):399-405. doi: 10.1247/csf.22.399.
Cultured Meth-A cells always include a small fraction of large cells, which had a DNA content above 4c (polyploid cells). The process from the formation to the disintegration of polyploid Meth-A cells was measured by means of time-lapse videography. Polyploid Meth-A cells arose spontaneously from normal cells (polyploidization), then died by apoptosis. The fraction of polyploid cells gradually increased in seven day-exponential cultures with a low concentration of demecolcine, which is a specific inhibitor of cell division. The results revealed that the polyploid Meth-A cells are generated from normal cells by failing cell division and that they die by apoptosis.
培养的Meth-A细胞总是包含一小部分大细胞，其DNA含量高于4c（多倍体细胞）。通过延时摄像测量多倍体Meth-A细胞从形成到解体的过程。多倍体Meth-A细胞由正常细胞自发产生（多倍体化），然后通过凋亡死亡。在含有低浓度秋水仙胺（一种细胞分裂特异性抑制剂）的七天指数培养物中，多倍体细胞的比例逐渐增加。结果表明，多倍体Meth-A细胞是由于细胞分裂失败而从正常细胞产生的，并且它们通过凋亡死亡。
