Orth M W, Chlebek K A, Cole A A, Schmid T M
Department of Animal Science, Michigan State University, East Lansing 48824, USA.
Res Vet Sci. 1997 Jul-Aug;63(1):11-4. doi: 10.1016/s0034-5288(97)90151-x.
Tetracyclines have been used extensively as antibiotics and growth promoters in the poultry industry. However, they can inhibit angiogenesis and matrix degradation, both of which are essential for normal growth plate cartilage development. The purpose of this research was to test the ability of several tetracyclines to inhibit cartilage degradation in cultured embryonic chick tibiae. Based on gross observations and biochemical quantitation of collagen release into the media, minocycline, doxycycline, oxytetracycline, and tetracycline inhibited cartilage degradation at 20, 40, 60, and 80 micrograms ml-1 respectively. Chlortetracycline did not inhibit cartilage degradation at concentrations tested. The ability of the tetracycline derivative to inhibit cartilage degradation was in general related to its hydrophobicity. Since a majority of the cartilage in the embryonic chick tibia will develop into the post hatched growth plate, it may be important to determine if any of the tetracyclines used as antibiotics could cause problems in in vivo growth plate cartilage development.
四环素在禽类养殖业中被广泛用作抗生素和生长促进剂。然而，它们会抑制血管生成和基质降解，而这两者对于正常生长板软骨发育至关重要。本研究的目的是测试几种四环素抑制培养的胚胎鸡胫骨软骨降解的能力。基于对培养基中胶原蛋白释放的大体观察和生化定量分析，米诺环素、多西环素、土霉素和四环素分别在20、40、60和80微克/毫升时抑制软骨降解。金霉素在所测试的浓度下未抑制软骨降解。四环素衍生物抑制软骨降解的能力总体上与其疏水性有关。由于胚胎鸡胫骨中的大部分软骨将发育成孵化后的生长板，确定用作抗生素的任何四环素是否会在体内生长板软骨发育中引起问题可能很重要。
