Rodríguez F, Fernández A, Ball H J
Department of Animal Pathology, Faculty of Veterinary Medicine, Las Palmas de Gran Canaria, Spain.
Res Vet Sci. 1997 Jul-Aug;63(1):91-2. doi: 10.1016/s0034-5288(97)90165-x.
A pool of three monoclonal antibodies, 3H12, 6D11 and 2A3, inhibited the in vitro growth of 12 Mycoplasma mycoides subspecies mycoides small colony (MmmSC) strains and seven Mycoplasma mycoides subspecies mycoides large colony (MmmLC) strains. This test did not cross-react with other 16 Mycoplasma mycoides cluster and nine non-Mycoplasma mycoides cluster strains representing 13 different species. Although MmmSC was not differentiated from MmmLC, the monoclonal antibodies distinguished both M mycoides subspecies mycoides biotypes from the other Mycoplasma species tested in contrast to results with polyclonal antisera.
一组三种单克隆抗体，即3H12、6D11和2A3，可抑制12株丝状支原体丝状亚种小菌落（MmmSC）菌株和7株丝状支原体丝状亚种大菌落（MmmLC）菌株的体外生长。该检测与其他16株丝状支原体簇菌株以及代表13个不同物种的9株非丝状支原体簇菌株无交叉反应。尽管MmmSC与MmmLC无法区分，但与多克隆抗血清的结果相反，这些单克隆抗体可将丝状支原体丝状亚种的两种生物型与所检测的其他支原体物种区分开来。
