Mitchelson K R, Cheng J, Kricka L J
ForBio Research Pty Ltd, Indooroopilly, Australia.
Trends Biotechnol. 1997 Nov;15(11):448-58. doi: 10.1016/S0167-7799(97)01117-7.
Advances in capillary electrophoresis technology over the past three years have been rapid. Capillary electrophoresis offers high-throughput, high-resolution, automatic operation and on-line detection with automatic data acquisition, and this has stimulated its application to the analysis of DNA mutations. Many different PCR-based DNA-mutation assays have been developed for unknown and known mutations. This article compares conventional PCR-based mutation-detection assays with the methods developed for use with capillary electrophoresis. Future trends for mutation detection using capillary electrophoresis are also assessed, with a special emphasis on totally integrated, microchip capillary-electrophoresis-based mutation-detection systems.
在过去三年中，毛细管电泳技术发展迅速。毛细管电泳具有高通量、高分辨率、自动操作以及可进行自动数据采集的在线检测功能，这推动了其在DNA突变分析中的应用。针对未知和已知突变，已经开发出了许多基于聚合酶链反应（PCR）的DNA突变检测方法。本文将基于PCR的传统突变检测方法与为配合毛细管电泳而开发的方法进行了比较。同时还评估了使用毛细管电泳进行突变检测的未来趋势，特别强调了基于全集成微芯片毛细管电泳的突变检测系统。
