Siegenthaler P A, Müller M O, Bovet L
Laboratoire de Physiologie Végétale, Université de Neuchâtel, Switzerland.
FEBS Lett. 1997 Oct 13;416(1):57-60. doi: 10.1016/s0014-5793(97)01168-x.
Three spinach chloroplast envelope membrane preparations (i.e. whole, outer and inner membranes) were incubated in the presence of [gamma-32P]ATP. After lipid extraction and separation by TLC, four main phosphorylated lipids were detected by autoradiography in whole envelope preparations. These phospholipids were identified by comparing their Rf with that of lipid markers and by a deacylation procedure. They were found to be phosphatidic acid (PA) and lyso-PA, L-alpha-phosphatidyl-inositol 4-monophosphate (PIP) and lyso-PIP. These lipids were not equally distributed in the outer and inner envelope membranes. Chloroplast envelope membranes were verified not to be contaminated by plasma membranes. It is concluded that lipid kinase activities are associated with spinach chloroplast envelope membranes.
将三种菠菜叶绿体被膜制剂（即完整膜、外膜和内膜）在[γ-32P]ATP存在的情况下进行孵育。脂质提取并通过薄层层析分离后，通过放射自显影在完整被膜制剂中检测到四种主要的磷酸化脂质。通过将它们的比移值与脂质标记物的比移值进行比较以及通过脱酰基程序来鉴定这些磷脂。发现它们是磷脂酸（PA）和溶血磷脂酸、L-α-磷脂酰肌醇4-单磷酸（PIP）和溶血PIP。这些脂质在外膜和内膜中的分布并不均匀。已证实叶绿体被膜未被质膜污染。得出的结论是，脂质激酶活性与菠菜叶绿体被膜相关。
