一种释放一氧化氮的试剂，S-亚硝基-N-乙酰青霉胺，可增强小鼠巨噬细胞系RAW264-7中超氧化物歧化酶mRNA的表达。
A nitric oxide-releasing reagent, S-nitroso-N-acetylpenicillamine, enhances the expression of superoxide dismutases mRNA in the murine macrophage cell line RAW264-7.
作者信息
Sano H, Hirai M, Saito H, Nakashima I, Isobe K I
机构信息
First Department of Internal Medicine, Nagoya University School of Medicine, Aichi, Japan.
出版信息
Immunology. 1997 Sep;92(1):118-22. doi: 10.1046/j.1365-2567.1997.00322.x.
Murine interferon-gamma (IFN-gamma) stimulates the murine macrophage tumour cell line RAW264-7 to produce nitric oxide (NO). IFN-gamma induces expression of inducible NO synthase (iNOS), manganese superoxide dismutase (Mn-SOD) and copper zinc SOD (CuZn-SOD) in these cells. To investigate the mechanism of induction of SOD expression, we added S-nitroso-N-acetyl penicillamine (SNAP) to RAW264-7 cells. SNAP enhanced the expression of Mn-SOD and CuZn-SOD. These results suggest that when producing NO, RAW264-7 cells express SOD that might protect them from NO toxicity.
小鼠γ干扰素（IFN-γ）刺激小鼠巨噬细胞肿瘤细胞系RAW264-7产生一氧化氮（NO）。IFN-γ诱导这些细胞中诱导型一氧化氮合酶（iNOS）、锰超氧化物歧化酶（Mn-SOD）和铜锌超氧化物歧化酶（CuZn-SOD）的表达。为了研究SOD表达的诱导机制，我们将S-亚硝基-N-乙酰青霉胺（SNAP）添加到RAW264-7细胞中。SNAP增强了Mn-SOD和CuZn-SOD的表达。这些结果表明，RAW264-7细胞在产生NO时表达SOD，这可能保护它们免受NO毒性的影响。
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