Wang L F, Rakela J, Laskus T
Division of Transplant Medicine, Pittsburgh Transplantation Institute, PA 15213, USA.
Mol Cell Probes. 1997 Oct;11(5):385-7. doi: 10.1006/mcpr.1997.0122.
Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.
有证据表明，半巢式PCR方案容易产生由多个头对尾引物串联重复组成的假象。在第二轮PCR中加入大量模板时，这种现象更为明显，并且仅限于第一轮和第二轮反应中使用的引物。
