Yasui M, Tanaka H, Seino Y
Department of Pediatrics, Okayama University Medical School, Japan.
Nephron. 1997;77(3):325-32. doi: 10.1159/000190296.
Programmed cell death (PCD) and cell type involving dying cells in the developing mouse and human fetal kidneys were investigated using the modified TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method in combination with immunohistochemistry. We identified many TUNEL-positive apoptotic cells mainly in the nephrogenic region in the developing mouse and human fetal kidneys. Tissue-fixed macrophages were widely distributed throughout the developing mouse and human fetal kidneys, including the nephrogenic zone. Most of the apoptotic cells in the nephrogenic zone were phagocytosed by tissue-fixed macrophages, suggesting that tissue-fixed macrophages play an important role in the process of PCD in the developing mouse kidney and human fetal kidney.
运用改良的TdT介导的dUTP生物素缺口末端标记法（TUNEL）并结合免疫组织化学技术，研究了发育中的小鼠和人类胎儿肾脏中程序性细胞死亡（PCD）以及涉及死亡细胞的细胞类型。我们发现，在发育中的小鼠和人类胎儿肾脏中，许多TUNEL阳性凋亡细胞主要位于肾发生区。组织固定的巨噬细胞广泛分布于整个发育中的小鼠和人类胎儿肾脏，包括肾发生带。肾发生区的大多数凋亡细胞被组织固定的巨噬细胞吞噬，这表明组织固定的巨噬细胞在发育中的小鼠肾脏和人类胎儿肾脏的PCD过程中发挥着重要作用。
