Kobayashi H, Orikasa M, Naito M, Kawasaki K, Oite T, Kawachi H, Yamashita A, Takeya M, Nihei H, Shimizu F
Department of Cell Biology, Institute of Nephrology, Niigata University School of Medicine, Japan.
Nephron. 1997;77(3):333-9. doi: 10.1159/000190297.
A phenotypic analysis of infiltrating macrophages in rat anti-Thy1 glomerulonephritis induced by monoclonal antibody (mAb) 1-22-3 was carried out using recently reported macrophage-specific mAbs. This was combined with a more detailed quantitative analysis, counting positive cells in isolated glomeruli, to obtain more information on the roles played by macrophages in glomerulonephritis. In normal glomeruli a small number of ED1- or OX-3(anti-Ia)-positive cells but almost no ED2-, TRPM-3- or Mar-3-positive cells were observed. ED1-positive cells increased from 2 h and peaked between days 3 and 7 after mAb injection. TRPM-3-positive cells increased from day 3 and peaked on day 7, later than ED1. The numbers of OX-3-positive cells changed in parallel with those of ED1-positive cells. Mar-3, which stained blood monocytes and ED2, which is an indicator oftissue-fixed resident macrophages, did not react with glomerular infiltrating macrophages. In a double staining study, about 40% of ED1- or OX-3-positive cells costained with TRPM-3 on day 3 and the percentage increased on day 7, but hardly any cells were positive for TRPM-3 alone. This results in two different phenotypes (ED1+,ED2-,OX-3+,Mar-3-, TRPM-3- and ED1+,ED2-,OX-3+,Mar-3-,TRPM-3+) of infiltrating macrophages. We conclude that in rat anti-Thy1 glomerulonephritis, monocytes/macrophages may infiltrate the mesangium, rapidly changing their phenotype (Mar-3+ to Mar-3-) and resulting in a gradual shift to TRPM-3-positive, activated macrophages.
利用最近报道的巨噬细胞特异性单克隆抗体，对单克隆抗体（mAb）1-22-3诱导的大鼠抗Thy1肾小球肾炎中浸润巨噬细胞进行了表型分析。这与更详细的定量分析相结合，即在分离的肾小球中计数阳性细胞，以获取更多关于巨噬细胞在肾小球肾炎中所起作用的信息。在正常肾小球中，观察到少量ED1或OX-3（抗Ia）阳性细胞，但几乎没有ED2、TRPM-3或Mar-3阳性细胞。注射mAb后，ED1阳性细胞从2小时开始增加，并在第3天至第7天达到峰值。TRPM-3阳性细胞从第3天开始增加，并在第7天达到峰值，比ED1出现峰值的时间晚。OX-3阳性细胞的数量与ED1阳性细胞的数量平行变化。Mar-3可对血液单核细胞染色，而ED2是组织固定驻留巨噬细胞的指标，它们均不与肾小球浸润巨噬细胞发生反应。在双重染色研究中，约40%的ED1或OX-3阳性细胞在第3天与TRPM-3共染色，且该百分比在第7天增加，但几乎没有细胞单独为TRPM-3阳性。这导致浸润巨噬细胞出现两种不同的表型（ED1+、ED2-、OX-3+、Mar-3-、TRPM-3-和ED1+、ED2-、OX-3+、Mar-3-、TRPM-3+）。我们得出结论，在大鼠抗Thy1肾小球肾炎中，单核细胞/巨噬细胞可能浸润系膜区，迅速改变其表型（从Mar-3+变为Mar-3-），并逐渐转变为TRPM-3阳性的活化巨噬细胞。
