Sondell M, Fex-Svenningsen A, Kanje M
Department of Animal Physiology, Lund University, Sweden.
Neuroreport. 1997 Sep 8;8(13):2871-6. doi: 10.1097/00001756-199709080-00014.
A combination of immunocytochemistry for glial specific antigens and bromodeoxyuridine (BrdU) and teasing was used to identify proliferating cells in cultured rat sciatic nerve segments. The nerve segments were exposed to insulin, or the insulin-like growth factors IGF-I and IGF-II. Teasing in combination with BrdU immunocytochemistry showed that around 93% of the proliferating cells in the nerve segments were Schwann cells. Immunostaining for BrdU and GFAP (glial fibrillary acid protein) showed that IGF-II enhanced proliferation of Schwann cells surrounding unmyelinated nerve fibres. In contrast, truncated IGF-I promoted proliferation of Schwann cells of myelinated nerve fibres while insulin increased proliferation of both cell types.
采用胶质特异性抗原免疫细胞化学、溴脱氧尿苷（BrdU）和神经纤维分离法相结合的方法，来鉴定培养的大鼠坐骨神经节段中的增殖细胞。将神经节段暴露于胰岛素、胰岛素样生长因子IGF-I和IGF-II中。神经纤维分离法与BrdU免疫细胞化学相结合显示，神经节段中约93%的增殖细胞为雪旺细胞。BrdU和GFAP（胶质纤维酸性蛋白）免疫染色显示，IGF-II增强了无髓神经纤维周围雪旺细胞的增殖。相比之下，截短的IGF-I促进了有髓神经纤维雪旺细胞的增殖，而胰岛素则增加了两种细胞类型的增殖。
