Ito K, Sass H, Urban J, Hofbauer A, Schneuwly S
Institut für Genetik, Universität Mainz, Saarstr. 21, D-55122 Mainz, Germany.
Cell Tissue Res. 1997 Oct;290(1):1-10. doi: 10.1007/s004410050901.
To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.
为了提高果蝇增强子陷阱和转基因品系中表达GAL4细胞的细胞质标记质量，我们测试了一种新的GAL4反应性报告基因UAS-tau，它在酵母上游激活序列（UAS）的控制下具有牛tau cDNA。Tau是一种微管相关蛋白，能主动且均匀地分布到所有细胞突起中。单克隆抗牛Tau抗体能详细显示标记细胞的轴突结构，其细节与高尔基浸染法相似。我们证明，UAS-tau系统对于研究胚胎后发育过程中已鉴定神经元的分化和重组过程特别有用。
