Manseau L, Baradaran A, Brower D, Budhu A, Elefant F, Phan H, Philp A V, Yang M, Glover D, Kaiser K, Palter K, Selleck S
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA.
Dev Dyn. 1997 Jul;209(3):310-22. doi: 10.1002/(SICI)1097-0177(199707)209:3<310::AID-AJA6>3.0.CO;2-L.
We have screened a collection of approximately 400 GAL4 enhancer trap lines for useful patterns of expression in the embryo, larval brain, imaginal discs, and ovary using a UAS-lacZ reporter construct. Although similar patterns of expression have previously been reported in the original P[lacZ] enhancer trap screens, these lines are useful for directing ectopic expression of genes in discrete patterns during these stages. In addition, we have identified some unique patterns of expression that have not been previously reported.
我们使用UAS-lacZ报告构建体，在胚胎、幼虫脑、成虫盘和卵巢中筛选了约400个GAL4增强子陷阱系，以寻找有用的表达模式。尽管在最初的P[lacZ]增强子陷阱筛选中曾报道过类似的表达模式，但这些品系对于在这些阶段以离散模式指导基因的异位表达很有用。此外，我们还鉴定出了一些以前未报道过的独特表达模式。
