Delgrossi M H, Breuza L, Mirre C, Chavrier P, Le Bivic A
Laboratoire de Génétique et Physiologie du Développement, Campus de Luminy, Marsaille, France.
J Cell Sci. 1997 Sep;110 ( Pt 18):2207-14. doi: 10.1242/jcs.110.18.2207.
To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of colon epithelial and Caco-2 cells. This apical localization was confirmed by confocal microscopy after transfection of the cDNA coding for either full length or N-terminally truncated human syntaxin 3 in Caco-2 cells. Furthermore the signal(s) and machinery targeting human syntaxin 3 to the apical membrane of epithelial cells are conserved between species since human syntaxin 3 was also localized at the apical membrane of canine MDCK cells and of epithelial cells in transgenic Drosophila melanogaster.
为了解人类肠道上皮细胞中顶端和基底外侧膜成分分选的分子机制，我们克隆了大鼠 syntaxin 3 的人类同源物，并研究了其亚细胞定位。发现内源性人类 syntaxin 3 定位于结肠上皮细胞和 Caco-2 细胞的顶端膜。在 Caco-2 细胞中转染编码全长或 N 端截短的人类 syntaxin 3 的 cDNA 后，共聚焦显微镜证实了这种顶端定位。此外，将人类 syntaxin 3 靶向上皮细胞顶端膜的信号和机制在物种间是保守的，因为人类 syntaxin 3 也定位于犬类 MDCK 细胞和转基因黑腹果蝇上皮细胞的顶端膜。
