Goureau O, Régnier-Ricard F, Jonet L, Jeanny J C, Courtois Y, Chany-Fournier F
Développement, Vieillissement et Pathologie de la Rétine, INSERM U 450, Association Claude Bernard, affiliée CNRS, Paris, France.
J Neurosci Res. 1997 Oct 1;50(1):104-13. doi: 10.1002/(SICI)1097-4547(19971001)50:1<104::AID-JNR11>3.0.CO;2-B.
During our studies on the multiple possible functions of nitric oxide (NO) in chick retinal development and physiology, we have demonstrated the presence and the activity of NO synthase (NOS-I and III) in certain neuronal populations (photoreceptors, amacrine cells in the inner nuclear and ganglion cells) and also in synaptic-rich regions in the developing chick retina. Both enzymes, detected by nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase, immunohistochemistry and Western blotting, appeared between embryonic days 6 and 12, and followed a spatial and temporal pattern of expression which correlated with the differentiation of the neuronal layers. Evaluation of the conversion of [3H]-labeled arginine to [3H]-citrulline, confirmed the presence of a calcium-dependent NOS activity in the cytosolic and particulate retinal extracts during the development. This pattern of NOS expression suggests that the regulated release of NO during key phases of development might be one mechanism involved in the regulation of retinal differentiation.
在我们对一氧化氮（NO）在雏鸡视网膜发育和生理学中多种可能功能的研究过程中，我们已经证明了一氧化氮合酶（NOS-I和III）在某些神经元群体（光感受器、内核层无长突细胞和神经节细胞）以及发育中的雏鸡视网膜富含突触的区域中的存在和活性。通过烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-黄递酶、免疫组织化学和蛋白质免疫印迹检测到的这两种酶，在胚胎第6天至12天之间出现，并遵循与神经元层分化相关的时空表达模式。对[3H]标记的精氨酸向[3H]瓜氨酸转化的评估，证实了在发育过程中，胞质和颗粒状视网膜提取物中存在钙依赖性一氧化氮合酶活性。这种一氧化氮合酶的表达模式表明，在发育的关键阶段，一氧化氮的调节释放可能是参与视网膜分化调节的一种机制。
