Nestoras K, Lee H, Mohler J
Department of Biological Sciences, Barnard College, New York, New York 10027, USA.
Genetics. 1997 Nov;147(3):1203-12. doi: 10.1093/genetics/147.3.1203.
We have undertaken a genetic analysis of new strong alleles of knot (kn). The original kn1 mutation causes an alteration of wing patterning similar to that associated with mutations of fused (fu), an apparent fusion of veins 3 and 4 in the wing. However, unlike fu, strong kn mutations do not affect embryonic segmentation and indicate that kn is not a component of a general Hh (Hedgehog)-signaling pathway. Instead we find that kn has a specific role in those cells of the wing imaginal disc that are subject to ptc-mediated Hh-signaling. Our results suggest a model for patterning the medial portion of the Drosophila wing, whereby the separation of veins 3 and 4 is maintained by kn activation in the intervening region in response to Hh-signaling across the adjacent anterior-posterior compartment boundary.
我们对“结瘤”（kn）的新的强等位基因进行了遗传分析。最初的kn1突变会导致翅脉模式的改变，类似于与“融合”（fu）突变相关的改变，即翅中第3和第4条翅脉明显融合。然而，与fu不同的是，强kn突变并不影响胚胎的体节形成，这表明kn不是一般的Hh（刺猬索尼克）信号通路的组成部分。相反，我们发现kn在翅成虫盘那些受到ptc介导的Hh信号作用的细胞中具有特定作用。我们的结果提出了一个果蝇翅内侧部分模式形成的模型，据此，在相邻的前后区室边界处，响应Hh信号，通过kn在中间区域的激活来维持第3和第4条翅脉的分离。
