Ellenberger T
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Chem Biol. 1995 Jun;2(6):351-4. doi: 10.1016/1074-5521(95)90213-9.
The recognition of abnormal DNA structure by proteins is fundamentally different from sequence-specific DNA binding. Recent crystal structures of uracil-DNA glycosylases show a novel uracil-binding pocket that explains the enzyme's selectivity for uracil-containing DNA and provide a structural basis for exploring the catalytic mechanism of amino-glycosylic bond cleavage.
蛋白质对异常DNA结构的识别与序列特异性DNA结合有着根本的不同。尿嘧啶-DNA糖基化酶的最新晶体结构显示了一个新的尿嘧啶结合口袋,这解释了该酶对含尿嘧啶DNA的选择性,并为探索氨基-糖苷键裂解的催化机制提供了结构基础。