Villegas A C, Kleven S H, Anderson D P
Avian Dis. 1976 Apr-Jun;20(2):342-54.
Mycoplasma gallisepticum and M. synoviae membranes were obtained by lysing mycoplasma cells with carbonate bicarbonate buffer at pH 10. Membrane preparations were compared with whole-cell preparations as to efficacy in the plate and tube agglutination and hemagglutination-inhibition tests. Membrane antigens were somewhat more sensitive in the plate and tube agglutination tests, whereas the hemagglutination-inhibition test showed no major difference between whole and membrane antigens. The membrane preparations were equal to whole-cell antigens in specificity.
通过用pH值为10的碳酸氢盐缓冲液裂解支原体细胞来获取鸡毒支原体和滑液支原体的细胞膜。在平板和试管凝集试验以及血凝抑制试验中，对细胞膜制剂与全细胞制剂的效力进行了比较。在平板和试管凝集试验中，膜抗原的敏感性略高，而血凝抑制试验显示全细胞抗原与膜抗原之间没有重大差异。细胞膜制剂在特异性方面与全细胞抗原相当。
