Zhang Q, Wu W X, Hong Ma X, Nathanielsz P W, Brenna J T
Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.
Prostaglandins Leukot Essent Fatty Acids. 1997 Sep;57(3):311-21. doi: 10.1016/s0952-3278(97)90550-0.
We report here changes in abundance of pulmonary cytosolic phospholipase A2 (cPLA2) and glucocorticoid receptor (GR) mRNAs and phospholipid (PL) fatty acids (FA) with gestational age, in either spontaneous or androstenedione-induced premature labor for 15 fetal rhesus monkeys and 1 neonate. Pulmonary RNA and lipids were extracted from the lungs of the rhesus monkeys, gestational ages ranging from 140 days to term. Northern hybridization analysis was performed for both cPLA2 and GR mRNAs. The results demonstrated the transcript of the cPLA2 constantly expressed and that of GR increased gradually in the lungs during the gestational stages studied. Phosphatidylcholine (PC), phosphatidylglycerol (PG) and phosphatidylinositol (PI) were separated by thin layer chromatography and the fatty acid composition in these PL was quantified by gas chromatography (GC). Regression analysis demonstrated that the expression of GR, mRNAs increased significantly with gestational age (P < 0.01). Furthermore, the level of GR mRNA is significantly correlated with that of cPLA2, with Pearson correlation coefficient of 0.83 (P < 0.01). The PC palmitate percentage increased significantly with gestational age (P < 0.01), while the PC unsaturation index and total polyunsaturated FA decreased during late gestation (P < 0.01). Changes in PG FA with gestational age were similar to those in PC, while PI FA did not change with gestational age. There was no effect of either spontaneous or induced labor on mRNA levels of cPLA2 and GR or the FA composition of pulmonary PL studied. We conclude that the pulmonary PL FA compositions are under developmental control.
我们在此报告了15只胎猴和1只新生猴在自然早产或雄烯二酮诱导的早产情况下，肺胞质型磷脂酶A2（cPLA2）和糖皮质激素受体（GR）mRNA丰度以及磷脂（PL）脂肪酸（FA）随胎龄的变化。从胎龄140天至足月的恒河猴肺中提取肺RNA和脂质。对cPLA2和GR mRNA进行Northern杂交分析。结果表明，在所研究的妊娠阶段，肺中cPLA2转录本持续表达，GR转录本逐渐增加。通过薄层层析分离磷脂酰胆碱（PC）、磷脂酰甘油（PG）和磷脂酰肌醇（PI），并通过气相色谱（GC）定量这些PL中的脂肪酸组成。回归分析表明，GR mRNA的表达随胎龄显著增加（P < 0.01）。此外，GR mRNA水平与cPLA2水平显著相关，Pearson相关系数为0.83（P < 0.01）。PC棕榈酸百分比随胎龄显著增加（P < 0.01），而PC不饱和指数和总多不饱和脂肪酸在妊娠后期下降（P < 0.01）。PG FA随胎龄的变化与PC相似，而PI FA不随胎龄变化。自然早产或诱导早产对所研究的cPLA2和GR mRNA水平或肺PL的FA组成均无影响。我们得出结论，肺PL FA组成受发育控制。
