生长激素与药物代谢。对大鼠肝脏微粒体混合功能氧化酶活性的急性影响。
Growth hormone and drug metabolism. Acute effects on microsomal mixed-function oxidase activities in rat liver.
作者信息
Wilson J T, Spelsberg T C
出版信息
Biochem J. 1976 Feb 15;154(2):433-8. doi: 10.1042/bj1540433.
Adult male rats were subjected either to sham operation or to hypophysectomy and adrenalectomy and maintained for a total of 10 days before treatment with growth hormone. Results of the early effects of growth hormone on the activities of the mixed-function oxidases in rat liver over a 96h period after growth-hormone treatment are presented. 2. Hypophysectomy and adrenalectomy result in decreased body and liver weight and decreased drug metabolism (mixed-function oxidases). Concentrations of electron-transport-system components are also decreased. 3. In the hypophysectomized/adrenalectomized rats, growth hormone decreases the activities of the liver mixed-function oxidases and the cytochrome P-450 and cytochrome c reductases, as well as decreasing the concentration of cytochrome P-450 compared with that of control rats. Similar but less dramatic results are obtained with sham-operated rats. 4. It is concluded that whereas growth hormone enhances liver growth, including induction of many enzyme activities, it results in a decrease in mixed-function oxidase activity. Apparently, mixed-function oxidase activity decreases in liver when growth (mitogenesis) increases.
成年雄性大鼠接受假手术或垂体切除及肾上腺切除，并在生长激素治疗前维持10天。文中呈现了生长激素治疗后96小时内对大鼠肝脏混合功能氧化酶活性的早期影响结果。2. 垂体切除及肾上腺切除导致体重和肝脏重量减轻以及药物代谢（混合功能氧化酶）降低。电子传递系统成分的浓度也降低。3. 在垂体切除/肾上腺切除的大鼠中，与对照大鼠相比，生长激素降低了肝脏混合功能氧化酶、细胞色素P-450和细胞色素c还原酶的活性，同时降低了细胞色素P-450的浓度。假手术大鼠也得到了类似但不太显著的结果。4. 得出的结论是，虽然生长激素促进肝脏生长，包括诱导许多酶活性，但它会导致混合功能氧化酶活性降低。显然，当生长（有丝分裂）增加时，肝脏中的混合功能氧化酶活性会降低。
Zotero 插件