使用各种环糊精作为添加剂对克罗米芬异构体进行毛细管电泳分离。

Capillary electrophoretic separation of clomiphene isomers using various cyclodextrins as additives.

作者信息

Juvancz Z, Urmös I, Klebovich I

机构信息

EGIS Pharmaceutical Ltd., Department of Pharmacokinetics, Budapest, Hungary.

出版信息

J Capillary Electrophor. 1996 Jul-Aug;3(4):181-9.

PMID:9384735

Abstract

The cis (Z) and trans (E) isomers of clomiphene were separated using capillary electrophoresis. Various derivatives of cyclodextrins (CDs) were applied as buffer additives, achieving a resolution of greater than 18. The effect of various parameters, type and concentration of CDs, type and concentration of background electrolytes (BGEs), pH, and organic modifiers has been studied systematically. Migration reversal was observed in several cases. The load ability of the various buffer systems was also taken into account.

摘要

使用毛细管电泳法分离了克罗米芬的顺式（Z）和反式（E）异构体。应用了各种环糊精（CDs）衍生物作为缓冲添加剂，实现了大于18的分离度。系统研究了各种参数的影响，包括CDs的类型和浓度、背景电解质（BGEs）的类型和浓度、pH值以及有机改性剂。在几种情况下观察到了迁移反转现象。还考虑了各种缓冲系统的负载能力。