Bashkin J S, Bartosiewicz M, Roach D, Leong J, Barker D, Johnston R
Molecular Dynamics, Sunnyvale, CA 94086, USA.
J Capillary Electrophor. 1996 Mar-Apr;3(2):61-8.
A capillary array electrophoresis (CAE) apparatus capable of running and analyzing DNA samples in 48 capillaries simultaneously has been constructed. The capillaries are individually replaceable, and sieving buffer can be easily pumped in and out of the capillary array as necessary. Samples are injected electrokinetically from polymerase chain reaction (PCR, Hoffmann-LaRoche, Nutley, NJ, U.S.A.) tubes arranged in a 6 x 8 format and are detected by laser-induced fluorescence. Data analysis software has been developed for semiautomatic analysis, including peak finding and DNA fragment sizing. The system represents a robust apparatus for the rapid and convenient analysis of DNA fragments in a high-throughout environment.
已构建出一种能够同时在48根毛细管中运行和分析DNA样本的毛细管阵列电泳（CAE）仪器。这些毛细管可单独更换，并且筛分缓冲液能够根据需要轻松地泵入和泵出毛细管阵列。样本从以6×8格式排列的聚合酶链反应（PCR，美国新泽西州纳特利市霍夫曼-罗氏公司）管中通过电动方式进样，并通过激光诱导荧光进行检测。已开发出用于半自动分析的数据分析软件，包括峰检测和DNA片段大小测定。该系统是一种强大的仪器，可在高通量环境中快速便捷地分析DNA片段。
