肌肉和成纤维细胞的肌动蛋白。
The actin of muscle and fibroblasts.
作者信息
Anderson P J
出版信息
Biochem J. 1976 May 1;155(2):297-301. doi: 10.1042/bj1550297.
Abstract
The isolation and quantification of an 18-residue peptide from the N-terminal region of chicken actin was used to quantify the amount of actin in acetone-dried powders of chicken breast muscle and chicken-embryo fibroblasts. Either isotope dilution or double labelling can be used for peptide quantification. About 17% of the protein of chicken breast muscle was estimated to be actin. However, only 0.25% of the protein of chicken-embryo fibroblasts was determined to be actin by quantification of this peptide. The actin content of fibroblasts may be low or the amino acid sequences of muscle and fibroblast actin may differ in the N-terminal region. The methodology used can be extended to examine whether other regions of muscle actin sequence are present in fibroblasts or other cell types.
摘要
从鸡肌动蛋白N端区域分离并定量一种18个残基的肽段，以此来定量鸡胸肌和鸡胚成纤维细胞丙酮干粉中的肌动蛋白含量。肽段定量可采用同位素稀释法或双标记法。据估计，鸡胸肌中约17%的蛋白质为肌动蛋白。然而，通过该肽段定量测定，鸡胚成纤维细胞中仅有0.25%的蛋白质被确定为肌动蛋白。成纤维细胞中的肌动蛋白含量可能较低，或者肌肉和成纤维细胞肌动蛋白的氨基酸序列在N端区域可能存在差异。所采用的方法可扩展用于检测成纤维细胞或其他细胞类型中是否存在肌肉肌动蛋白序列的其他区域。
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本文引用的文献
1
ACTIN: A COMPARATIVE STUDY.肌动蛋白：一项比较研究。
Biochim Biophys Acta. 1964 Sep 4;90:534-41. doi: 10.1016/0304-4165(64)90232-6.
2
Quantitative studies on the structure of cross-striated myofibrils. II. Investigations by biochemical techniques.横纹肌肌原纤维结构的定量研究。II. 生化技术研究
Biochim Biophys Acta. 1957 Feb;23(2):250-60. doi: 10.1016/0006-3002(57)90326-8.
3
One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells.西部马脑炎病毒在体外培养的鸡胚细胞上的一步生长曲线及单细胞病毒产量分析
J Exp Med. 1954 Feb;99(2):183-99. doi: 10.1084/jem.99.2.183.
4
Amino acid sequence homology in the active site of rabbit and sturgeon muscle aldolases.兔和鲟鱼肌肉醛缩酶活性位点的氨基酸序列同源性。
FEBS Lett. 1970 Sep 18;10(1):49-53. doi: 10.1016/0014-5793(70)80413-6.
5
A comparative study of the structure of muscle fructose 1,6-diphosphate aldolases.肌肉果糖1,6 - 二磷酸醛缩酶结构的比较研究。
Eur J Biochem. 1969 Dec;11(3):503-9. doi: 10.1111/j.1432-1033.1969.tb00802.x.
6
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J Mol Biol. 1973 Jun 25;77(2):279-90. doi: 10.1016/0022-2836(73)90336-7.
7
Aging effects on the liver aldolase of rabbits.衰老对家兔肝脏醛缩酶的影响。
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