颌下腺和胰腺中转化生长因子-α（TGF-α）表达的种属差异。

Species differences in the expression of transforming growth factor-alpha (TGF-alpha) in the submandibular gland and pancreas.

作者信息

Ikematsu Y, Pour P M, Kazakoff K

机构信息

UNMC/Eppley Cancer Center, University of Nebraska Medical Center, Omaha 68198-6805, USA.

出版信息

Int J Pancreatol. 1997 Oct;22(2):111-9. doi: 10.1007/BF02787468.

DOI:10.1007/BF02787468

PMID:9387032

Abstract

CONCLUSION

Significant differences exist in the expression of transforming growth factor-alpha (TGF-alpha) in the submandibular glands (SMG) and the pancreas of different species and among cell components in the same species.

BACKGROUND

Our previous studies have shown marked differences in the expression of TGF-alpha in the pancreas of humans and Syrian hamsters. To examine whether these differences also exist in other species, we examined the expression of TGF-alpha in the pancreas of mouse, rat, Syrian hamster, guinea pig, rabbit, pig, dog, and monkey. We included the SMG of these species for comparison.

MATERIALS AND METHODS

The formalin-fixed tissues of these species (n = 3) were investigated by immuno-histochemistry using a monoclonal antibody to TGF-alpha. The SMG of rat, mouse, hamster, rabbit, pig, dog, and monkey were examined by RT-PCR to assure the specificity of the antibody.

RESULTS

Remarkable species differences were found in the expression of this peptide in both the SMG and the pancreas. In the SMG, the expression varied in different cell components, even in the same tissue of the species. Although excretory and secretory ducts of the SMG of most species reacted with the antibody, intercalated ducts were immunoreactive only in mouse and guinea pig. Acinar cells were either weakly positive or nonimmunoreactive. In the pancreas of most species, the cells of the large and medium-sized ducts expressed TGF-alpha, whereas centroacinar cells of only rat and dog reacted with the antibody. Marked differences were found in the expression of TGF-alpha in islet cells and in its spatial distribution. Differences were also found in the immunoreactivity of mesenchymal and neural cells among the species.

摘要

结论

不同物种的颌下腺（SMG）和胰腺中，以及同一物种的不同细胞成分中，转化生长因子-α（TGF-α）的表达存在显著差异。

背景

我们之前的研究表明，人类和叙利亚仓鼠胰腺中TGF-α的表达存在显著差异。为了研究这些差异是否也存在于其他物种中，我们检测了小鼠、大鼠、叙利亚仓鼠、豚鼠、兔子、猪、狗和猴子胰腺中TGF-α的表达。我们还纳入了这些物种的颌下腺进行比较。

材料和方法

使用抗TGF-α单克隆抗体，通过免疫组织化学法对这些物种（n = 3）的福尔马林固定组织进行研究。通过逆转录聚合酶链反应（RT-PCR）检测大鼠、小鼠、仓鼠、兔子、猪、狗和猴子的颌下腺，以确保抗体的特异性。

结果

在颌下腺和胰腺中，该肽的表达均存在显著的物种差异。在颌下腺中，即使在同一物种的同一组织中，不同细胞成分的表达也有所不同。尽管大多数物种颌下腺的排泄管和分泌管与抗体发生反应，但闰管仅在小鼠和豚鼠中有免疫反应性。腺泡细胞要么弱阳性，要么无免疫反应性。在大多数物种的胰腺中，大中型导管的细胞表达TGF-α，而只有大鼠和狗的中央腺泡细胞与抗体发生反应。胰岛细胞中TGF-α的表达及其空间分布存在显著差异。不同物种间的间充质细胞和神经细胞的免疫反应性也存在差异。