Ishikawa O, Ohigashi H, Imaoka S, Nakai I, Mitsuo M, Weide L, Pour P M
Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Am J Pathol. 1995 Nov;147(5):1456-64.
Our previous studies have suggested that the presence of intact islets is essential for the induction of pancreatic exocrine tumors in the Syrian hamster model. To validate this, we investigated the effect of the carcinogen, N-nitrosobis(2-oxo-propyl)amine (BOP) in hamsters, in which homologous isolated intact islets were transplanted into the submandibular gland (SMG). Freshly isolated pure islets from hamster donors were transplanted into the left SMG of 20 female host hamsters. Ten of these hamsters (group 1) received BOP (40 mg/kg) weekly for 3 weeks. Another 10 hamsters (group 2) were kept untreated. In groups 3 and 4 (10 hamsters each) the salt solution or isolated pancreatic ductal cells, respectively, was injected into the gland. In other groups (10 hamsters each) islets were transplanted into the peri-SMG connective tissue (group 5) or into the renal subcapsular space (group 6). Hamsters of group 1 (40 mg/kg, weekly for 3 weeks) as were group 7 hamsters, which served as BOP-treated controls. All BOP-treated hamsters developed pancreatic lesions. Similar hyperplastic and atypical ductal/ductular proliferation and in situ carcinoma were found in the SMG of many group 1 hamsters. No such lesions were found in the SMG, peri-SMG, or renal subcapsular space of the other groups. Islets appear to be involved in carcinogenicity of BOP. The mechanism is obscure.
我们之前的研究表明，完整胰岛的存在对于叙利亚仓鼠模型中胰腺外分泌肿瘤的诱导至关重要。为了验证这一点，我们研究了致癌物N-亚硝基双（2-氧代丙基）胺（BOP）对仓鼠的影响，其中将同源分离的完整胰岛移植到下颌下腺（SMG）中。将来自仓鼠供体的新鲜分离的纯胰岛移植到20只雌性宿主仓鼠的左下颌下腺中。其中10只仓鼠（第1组）每周接受BOP（40 mg/kg），共3周。另外10只仓鼠（第2组）不进行处理。在第3组和第4组（每组10只仓鼠）中，分别将盐溶液或分离的胰腺导管细胞注入腺体。在其他组（每组10只仓鼠）中，将胰岛移植到下颌下腺周围结缔组织（第5组）或肾被膜下间隙（第6组）。第1组仓鼠（40 mg/kg，每周3周）以及作为BOP处理对照的第7组仓鼠均出现胰腺病变。在许多第1组仓鼠的下颌下腺中发现了类似的增生性和非典型导管/小导管增殖以及原位癌。在其他组的下颌下腺、下颌下腺周围或肾被膜下间隙中未发现此类病变。胰岛似乎参与了BOP的致癌作用。其机制尚不清楚。
