Lai W, Zhao M, Luo S
School of Stomatology, West China University of Medical Sciences, Chengdu.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1996 Mar;31(2):91-4.
The purpose of this study is to evaluate insulin change in condylar cartilage during functional advancement of rat mandible by immunohistochemical technique (ABC method) in order to provide further information about the role of insulin in orthopedics and the relationship between insulin and proliferation of condylar cartilage. Staining gradient of insulin is observed in condylar cartilage, with the stain deepest in the geriminal layer, medial in the transitional, least in the maturational layer. The posterior region of condylar cartilage in the experimental groups is shown to have thickened significantly as compared with the control groups, especially the germinal layer. Stained-positive cells exhibit deeper stain and higher percentage. The stain in the other layers is also more obvious than that in the control groups. The results suggest: Insulin level in condyle changes with the different cartilage layers. Insulin distribution appears higher when condylar chondrocytes proliferate rapidly and lower when they differentiate actively. Functional orthopedics may stimulate the growth of condylar cartilage and raise the insulin level. The demonstrates that insulin correlates with hyperplasia of conylar chondrocytes.
本研究的目的是通过免疫组织化学技术（ABC法）评估大鼠下颌骨功能前移过程中髁突软骨内胰岛素的变化，以便为胰岛素在骨科中的作用以及胰岛素与髁突软骨增殖之间的关系提供更多信息。在髁突软骨中观察到胰岛素的染色梯度，生发层染色最深，过渡层次之，成熟层最少。与对照组相比，实验组髁突软骨后区明显增厚，尤其是生发层。染色阳性细胞染色更深，百分比更高。其他层的染色也比对照组更明显。结果表明：髁突内胰岛素水平随软骨层不同而变化。当髁突软骨细胞快速增殖时，胰岛素分布较高；当它们积极分化时，胰岛素分布较低。功能性正畸可能刺激髁突软骨生长并提高胰岛素水平。这表明胰岛素与髁突软骨细胞增生相关。
