Shi H, Zhong X, Xu H
Department of Respiratory Medicine, First Affiliated Hospital, Guangxi Medical University, Nanning.
Zhonghua Nei Ke Za Zhi. 1996 Jun;35(6):371-4.
Repeated aerosolized ovalbumin (OVA) challenge of OVA-sensitized mice induces bronchoalveolar lavage fluid (BALF) eosinophilia. The influence of interferon-gamma (IFN-gamma) on antigen-induced eosinophil recruitment in the airway as well as the concentration of interleukin-5 (IL-5) in supernatants of cultured spleen cells were examined. In sensitized mice challenged with OVA 20 minutes once a day for 6 days, the number of BALF eosinophils was (9.53 +/- 0.84) x 10(8)/L. However, no eosinophil could be found in the BALF from mice without OVA sensitization and challenge. In mice treated with intraperitoneal injection of INF-gamma before each challenge, different doses of IFN-gamma (1.0 x 10(3) U/kg, 1.0 x 10(4) U/kg and 1.0 x 10(5) U/kg) produced different degrees of eosinophilia inhibition 29.8% (P < 0.05), 55.7% (P < 0.01) and 69.0% (P < 0.01). Our results showed that IFN-gamma prevented antigen-induced eosinophil infiltration in airway accompanied by a decrement of levels of IL-5 with a dose related response. It is concluded that IFN-gamma is capable of inhibiting eosinophil infiltration in mouse airway by inhibiting the production of IL-5. Our results suggested that IFN-gamma may be of value in eliminating asthmatic airway inflammation in humans.
对卵清蛋白（OVA）致敏的小鼠反复进行雾化OVA激发可诱导支气管肺泡灌洗液（BALF）嗜酸性粒细胞增多。研究了干扰素-γ（IFN-γ）对气道中抗原诱导的嗜酸性粒细胞募集的影响以及培养的脾细胞上清液中白细胞介素-5（IL-5）的浓度。在每天一次用OVA激发20分钟，共6天的致敏小鼠中，BALF嗜酸性粒细胞数量为（9.53±0.84）×10⁸/L。然而，在未进行OVA致敏和激发的小鼠的BALF中未发现嗜酸性粒细胞。在每次激发前腹腔注射INF-γ治疗的小鼠中，不同剂量的IFN-γ（1.0×10³U/kg、1.0×10⁴U/kg和1.0×10⁵U/kg）产生了不同程度的嗜酸性粒细胞增多抑制，分别为29.8%（P<0.05）、55.7%（P<0.01）和69.0%（P<0.01）。我们的结果表明，IFN-γ可预防抗原诱导的气道嗜酸性粒细胞浸润，并伴有IL-5水平的剂量相关下降。结论是IFN-γ能够通过抑制IL-5的产生来抑制小鼠气道中的嗜酸性粒细胞浸润。我们的结果提示IFN-γ可能对消除人类哮喘气道炎症有价值。
