Wang H, Zhang J, Xu Y, Wei Q, Liu S
Institute of Basic Medical Sciences, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1996 Aug;18(4):246-51.
A replication-defective retrovirus vector containing the human beta-globin gene and 36-base-pair HS2 enhancer (N2A beta E36) was constructed and transferred into psi-2 and PA317 packaging cell lines. Titers of amphotropic recombinant retrovirus ranged from 10(3) to 10(4) CFU/ml. Southern blot analysis indicated that intact human beta-globin gene was stably integrated into the genome of the target cells. Northern blot hybridization analysis showed that human beta-globin gene could be expressed in mouse fetal liver cells.
构建了一种含有人类β-珠蛋白基因和36个碱基对的HS2增强子的复制缺陷型逆转录病毒载体（N2AβE36），并将其转入psi-2和PA317包装细胞系。嗜异性重组逆转录病毒的滴度范围为10³至10⁴CFU/ml。Southern印迹分析表明，完整的人类β-珠蛋白基因稳定整合到靶细胞的基因组中。Northern印迹杂交分析表明，人类β-珠蛋白基因可在小鼠胎儿肝细胞中表达。
