Liu M, Zheng S
Institute of Basic Medical Sciences, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1996 Jun;18(3):166-71.
In this study, two high-efficiency eukaryotic expression vectors, PED35 and PED40 containing p35 and p40 cDNA of rhIL-12 respectively, were co-transfected into COS-7 cells by modified DEAE-Dextran method. The COS-7 cell culture supernatant had been certified to contain high level of expressed rhIL-12 protein, nearly 600 IU/ml. Furthermore, in the two biological assays, the expressed rhIL-12 protein can cause the proliferation of human PHA-activated lymphoblasts and augmentation of NK cells mediated cytotoxic activity. This study, therefore, could help further exploration of biological activities and potential usage of rhIL-12.
在本研究中，分别含有重组人白细胞介素-12（rhIL-12）的p35和p40 cDNA的两种高效真核表达载体PED35和PED40，通过改良的DEAE-葡聚糖法共转染至COS-7细胞中。COS-7细胞培养上清液已被证实含有高水平表达的rhIL-12蛋白，接近600 IU/ml。此外，在两项生物学检测中，表达的rhIL-12蛋白可导致人PHA激活的淋巴母细胞增殖，并增强NK细胞介导的细胞毒活性。因此，本研究有助于进一步探索rhIL-12的生物学活性和潜在用途。
