Kirkwood L C, Nation R L, Somogyi A A
Centre for Pharmaceutical Research, School of Pharmacy and Medical Sciences, University of South Australia, North Terrace, Adelaide, Australia.
J Chromatogr B Biomed Sci Appl. 1997 Nov 7;701(1):129-34. doi: 10.1016/s0378-4347(97)00354-x.
A high-performance liquid chromatographic assay for the oxidative metabolites of dihydrocodeine, nordihydrocodeine and dihydromorphine, formed in human liver microsomal incubations, is described. A simple solvent extraction followed by reversed-phase high-performance liquid chromatography with UV detection allows quantification of both metabolites in a single assay. Standard curve concentration ranges for dihydromorphine and nordihydrocodeine were 0.05-5 and 0.2-20 microM, respectively. Assay performance was assessed by intra- and inter-day accuracy and precision of quality control (QC) samples. The difference between the calculated and the actual concentration and the relative standard deviation were less than 15% at low QC concentrations and less than 10% at medium and high QC concentrations for both analytes. The method provides good precision, accuracy and sensitivity for use in kinetic studies of the oxidative metabolism of dihydrocodeine in human liver microsomes.
本文描述了一种用于检测二氢可待因、去甲二氢可待因和二氢吗啡在人肝微粒体孵育中形成的氧化代谢产物的高效液相色谱法。通过简单的溶剂萃取，然后采用带有紫外检测的反相高效液相色谱法，可在单次检测中对两种代谢产物进行定量。二氢吗啡和去甲二氢可待因的标准曲线浓度范围分别为0.05 - 5微摩尔/升和0.2 - 20微摩尔/升。通过质量控制（QC）样品的日内和日间准确度及精密度评估检测性能。对于两种分析物，在低QC浓度下，计算浓度与实际浓度的差值以及相对标准偏差均小于15%；在中、高QC浓度下，均小于10%。该方法为二氢可待因在人肝微粒体中的氧化代谢动力学研究提供了良好的精密度、准确度和灵敏度。
