Iwahashi M, Ooshima A, Nakano R
Department of Obstetrics and Gynaecology, Wakayama Medical College, Japan.
J Clin Pathol. 1997 Sep;50(9):755-9. doi: 10.1136/jcp.50.9.755.
To obtain insight into the effects of oestrogen on extracellular matrix (ECM) in the postmenopausal endometrium.
The distribution of the components of the ECM, including collagen types I, III, IV, and VI, and laminin, was investigated in the human postmenopausal endometrium by an indirect immunofluorescence method with specific monoclonal antibodies and a polyclonal antibody. Collagens were also extracted from the endometrial tissues of postmenopausal women who had or had not been treated with oestrogen for three weeks.
Immunohistochemical studies demonstrated that type I collagen was the predominant interstitial collagen, and that types III and VI collagens were absent or very sparsely distributed in the stroma of the postmenopausal endometrium. However, types I, III, and VI collagens were diffusely localised in the stroma of the postmenopausal endometrium after administration of oestrogen. Even though type IV collagen was not seen in the basement membrane of the endometrial glands in the endometrium of postmenopausal women in the absence of oestrogen treatment, both type IV collagen and laminin were localised exclusively in the basement membrane of the endometrial glands in the postmenopausal endometrium after three weeks of oestrogen treatment. The level of type III collagen relative to that of type I collagen was significantly increased (p < 0.01) in the endometrium of oestrogen treated postmenopausal women compared with non-treated postmenopausal women.
Conjugated equine oestrogen might induce changes in the distribution of components and in the composition of the ECM in the endometrium of postmenopausal women.
深入了解雌激素对绝经后子宫内膜细胞外基质（ECM）的影响。
采用特异性单克隆抗体和多克隆抗体的间接免疫荧光法，研究绝经后妇女子宫内膜中细胞外基质成分（包括I、III、IV和VI型胶原蛋白以及层粘连蛋白）的分布情况。还从接受或未接受雌激素治疗三周的绝经后妇女的子宫内膜组织中提取胶原蛋白。
免疫组织化学研究表明，I型胶原蛋白是主要的间质胶原蛋白，III型和VI型胶原蛋白在绝经后子宫内膜基质中不存在或分布非常稀少。然而，给予雌激素后，I、III和VI型胶原蛋白在绝经后子宫内膜基质中呈弥漫性分布。在未接受雌激素治疗的绝经后妇女子宫内膜中，子宫内膜腺体基底膜中未见IV型胶原蛋白，但在雌激素治疗三周后的绝经后子宫内膜中，IV型胶原蛋白和层粘连蛋白均仅定位于子宫内膜腺体的基底膜。与未治疗的绝经后妇女相比，接受雌激素治疗的绝经后妇女子宫内膜中III型胶原蛋白相对于I型胶原蛋白的水平显著升高（p < 0.01）。
结合马雌激素可能会引起绝经后妇女子宫内膜中细胞外基质成分分布和组成的变化。
