Corey H E, Alfonso F, Hamele-Bena D, Greenstein S M, Schechner R, Tellis V, Geva P, Koss L G
Department of Pediatrics, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, New York, USA.
Acta Cytol. 1997 Nov-Dec;41(6):1742-6. doi: 10.1159/000333178.
Urine immunocytology may provide a noninvasive method of investigating the antigens expressed by renal tubular cells. In previous investigations of patients with acute renal allograft rejection (AR), we showed that the adhesion molecule ICAM-1 is expressed by voided tubular cells. The up-regulation of ICAM-1, in turn, may be due to high circulating levels of interferon-gamma and/or TNF-alpha. We investigated the regulation of receptors for these cytokines and found a correlation between their expression and clinical events.
For 10 patients who received transplants consecutively, freshly voided aliquots of urine were obtained on each hospital day and on each outpatient visit for a mean of 52.8 +/- 26.2 (SD) days. After cytocentrifugation, the samples were prepared by the avidin-biotin-immunoperoxidase technique in order to detect the presence or absence of ICAM-1, interferon-gamma receptor and TNF-alpha receptor (p 80) on the tubular cells.
In nonrejecting patients, the tubular cells expressed the interferon-gamma receptor but not ICAM-1 or the TNF-alpha receptor. In patients with AR, the pattern was different. The tubular cells expressed ICAM-1 and the TNF-alpha receptor but not the interferon-gamma receptor.
Urine immunocytochemistry may be useful to demonstrate the expression of cytokine receptors by renal epithelia.
尿免疫细胞学可为研究肾小管细胞表达的抗原提供一种非侵入性方法。在先前对急性肾移植排斥反应（AR）患者的研究中，我们发现脱落的肾小管细胞表达黏附分子ICAM - 1。而ICAM - 1的上调可能归因于循环中高水平的干扰素 - γ和/或肿瘤坏死因子 - α。我们研究了这些细胞因子受体的调节情况，并发现它们的表达与临床事件之间存在相关性。
对于10例连续接受移植的患者，在每个住院日以及每次门诊就诊时均采集新鲜晨尿样本，共采集了平均52.8±26.2（标准差）天。经细胞离心后，采用抗生物素蛋白 - 生物素 - 免疫过氧化物酶技术制备样本，以检测肾小管细胞上ICAM - 1、干扰素 - γ受体和肿瘤坏死因子 - α受体（p80）的有无。
在未发生排斥反应的患者中，肾小管细胞表达干扰素 - γ受体，但不表达ICAM - 1或肿瘤坏死因子 - α受体。在发生AR的患者中，情况则不同。肾小管细胞表达ICAM - 1和肿瘤坏死因子 - α受体，但不表达干扰素 - γ受体。
尿免疫细胞化学可能有助于证明肾上皮细胞中细胞因子受体的表达。
