Bock M J, Bara I, LeDonne N, Martz A, Dyroff M
Zeneca Pharmaceuticals, Wilmington, DE 19850-5437, USA.
J Chromatogr B Biomed Sci Appl. 1997 Oct 24;700(1-2):131-8. doi: 10.1016/s0378-4347(97)00129-1.
An assay was developed for the quantification of anastrozole [2,2'-[5-(1H-1,2,4-triazol-1-ymethyl)-1,3-phenylene]bis(2-++ +methylpropiononitrile)] in human plasma using liquid-liquid extraction. Anastrozole and an internal standard were chromatographed and detected by gas chromatography with electron capture detection, using a combination temperature-pressure program. The range of the assay is 3 to 100 ng/ml. Anastrozole was quantified by comparing its peak area to that of an internal standard. A cross-validation of this assay was also successfully performed between several laboratories.
