采用微径液相色谱结合紫外和荧光检测法测定血液和肌肉微透析样品中的SDZ ICM 567。
Determination of SDZ ICM 567 in blood and muscle microdialysis samples by microbore liquid chromatography with ultraviolet and fluorescence detection.
作者信息
Ofner B, Boukhabza A, Pacha W, Amsterdam C V, Wintersteiger R
机构信息
Institute of Pharmaceutical Chemistry, Graz, Austria.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Oct 24;700(1-2):191-200. doi: 10.1016/s0378-4347(97)00247-8.
Fast, simple and accurate methods for the determination of SDZ ICM 567, the 7-methoxy derivative of tropisetron, in microdialysates have been developed. Sampling by microdialysis from freely moving rats in the portal and jugular vein offers a new technology for pharmacokinetic studies by direct and continuous measurement of unbound drug concentrations with time. SDZ ICM 567 can be identified in small sample volumes of dialysates on a microbore high-performance liquid chromatography column-switching system with ultraviolet detection. In addition, determination of SDZ ICM 567 by fluorimetric detection has been developed for muscle microdialysates from rats. [14C]SDZ ICM 567 was used as reference substance for the estimation of the amount of substance transferred through the dialysis membrane. The radioactive measurement (RA) gave the recovery information, whereas the liquid chromatographic method detected the sum of [14C]SDZ ICM 567 and dialyzed SDZ ICM 567.
已经开发出快速、简单且准确的方法来测定微透析液中的SDZ ICM 567（托烷司琼的7-甲氧基衍生物）。通过微透析对自由活动大鼠的门静脉和颈静脉进行采样，为药代动力学研究提供了一种新技术，可直接且连续地随时间测量未结合药物浓度。SDZ ICM 567可在配备紫外检测的微径高效液相色谱柱切换系统上，从小体积的透析液样本中进行鉴定。此外，还开发了用于大鼠肌肉微透析液的荧光检测法来测定SDZ ICM 567。[14C]SDZ ICM 567用作参考物质，用于估算透过透析膜转移的物质量。放射性测量（RA）给出回收率信息，而液相色谱法检测[14C]SDZ ICM 567和透析出的SDZ ICM 567的总和。
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