Duggal R, Cuconati A, Gromeier M, Wimmer E
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA.
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13786-91. doi: 10.1073/pnas.94.25.13786.
Genetic recombination of plus-strand RNA viruses is an important process for promoting genetic variation. By using genetically marked poliovirus RNAs, we have demonstrated that genetic recombination can occur in a cell-free system that generates infective virus from added poliovirus RNA. Recombinant polioviruses were isolated, and the region of crossing over was roughly mapped. Recombinants could be isolated even under conditions where the yield of viruses from one of the parental RNAs was depressed to levels comparable to or less than the yield of recombinant viruses, an observation suggesting that only one of the recombining RNAs needs to be replication-competent. The generation of poliovirus recombinants in a cell-free system offers new possibilities for studying recombination and evolution of RNA viruses.
正链RNA病毒的基因重组是促进基因变异的重要过程。通过使用基因标记的脊髓灰质炎病毒RNA，我们已经证明基因重组可以在无细胞系统中发生，该系统能从添加的脊髓灰质炎病毒RNA产生感染性病毒。分离出了重组脊髓灰质炎病毒，并大致绘制了交叉区域。即使在一种亲本RNA的病毒产量降低到与重组病毒产量相当或更低水平的条件下，仍能分离出重组体，这一观察结果表明，只有一种参与重组的RNA需要具备复制能力。在无细胞系统中产生脊髓灰质炎病毒重组体为研究RNA病毒的重组和进化提供了新的可能性。
