Kirkegaard K, Baltimore D
Cell. 1986 Nov 7;47(3):433-43. doi: 10.1016/0092-8674(86)90600-8.
We have investigated RNA recombination among poliovirus genomes by analyzing both intratypic and intertypic recombinant crosses involving the same defined genetic markers. Sequence analysis of the recombinant junctions of 13 nonsibling intertypic recombinants showed that intertypic RNA recombination is not site-specific, nor does it require extensive homology between the recombining parents at the crossover site. To discriminate between breaking-rejoining and copy choice mechanisms of RNA recombination, we have inhibited the replication of the recombining parents independently and found opposite effects on the frequency of genetic recombination in intratypic crosses. The results strongly support a copy choice mechanism for RNA recombination, in which the viral RNA polymerase switches templates during negative strand synthesis.
我们通过分析涉及相同明确遗传标记的同型和异型重组杂交，对脊髓灰质炎病毒基因组间的RNA重组进行了研究。对13个非同族异型重组体的重组连接点进行序列分析表明，异型RNA重组不是位点特异性的，在交叉位点处重组亲本之间也不需要广泛的同源性。为了区分RNA重组的断裂-重新连接和模板选择机制，我们分别抑制了重组亲本的复制，并发现对同型杂交中基因重组频率有相反的影响。结果有力地支持了RNA重组的模板选择机制，即病毒RNA聚合酶在负链合成过程中切换模板。
