Tsukaya H, Uchimiya H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Mol Gen Genet. 1997 Oct;256(3):231-8. doi: 10.1007/s004380050565.
Developmental control of the formation of the serrated margin of leaf blades was investigated. First, the expression was characterized of a marker gene encoding beta-glucuronidase in strain #1-35-38, a transgenic strain of Arabidopsis thaliana (L.) Heynh, derived by the use of a previously described transposon-tagging system. In strain #1-35-38, expression of the marker gene was tissue-specific, being restricted to stipules and the toothed margins of laminae. Using this transgenic marker gene, we examined the development of leaf blade margins in Arabidopsis. We compared the pattern of expression of the marker gene in the leaves of the wild-type plant with that in plants carrying the asymmetric leaves1 (as1) mutation, which causes dramatic changes in leaf-blade morphology in Arabidopsis. The as1 mutant showed normal morphology of early leaf primordia. The mutation affected the development of leaf segmentation in Arabidopsis without any change in the number or morphology of cells in laminae. The as1 mutation affected leaf morphology independently of mutations in other genes known to affect leaf morphogenesis, such as the acaulis1 mutation and the angustifolia mutation. Based upon these results, the development of the morphology of leaf margins in Arabidopsis is discussed.
对叶片锯齿状边缘形成的发育控制进行了研究。首先，对编码β-葡萄糖醛酸酶的标记基因在#1-35-38株系中的表达进行了表征，该株系是利用先前描述的转座子标签系统获得的拟南芥转基因株系。在#1-35-38株系中，标记基因的表达具有组织特异性，仅限于托叶和叶片的齿状边缘。利用这个转基因标记基因，我们研究了拟南芥叶片边缘的发育。我们比较了野生型植物叶片与携带不对称叶片1（as1）突变的植物叶片中标记基因的表达模式，该突变会导致拟南芥叶片形态发生显著变化。as1突变体早期叶原基形态正常。该突变影响了拟南芥叶片分割的发育，而叶片中细胞的数量或形态没有任何变化。as1突变独立于其他已知影响叶片形态发生的基因突变，如无茎1突变和狭叶突变，对叶片形态产生影响。基于这些结果，对拟南芥叶片边缘形态的发育进行了讨论。
