Larsen T, Andersen H K, Fiehn N E
Department of Oral Microbiology, School of Dentistry, University of Copenhagen, Denmark.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997 Nov;84(5):513-6. doi: 10.1016/s1079-2104(97)90268-7.
Dental high-speed turbines and handpieces can take up and expel microorganisms during operation and thus need regular sterilization. This study established a method for validating devices used to sterilize high-speed turbines and handpieces. The air and water channels and turbine chambers were contaminated with suspensions of Streptococcus salivarius or endospores of Bacillus stearothermophilus. The effect of flushing and/or autoclaving performed by a new device combining both procedures was evaluated by counting the number of viable bacteria recovered from these devices. Further, the effect on clinically used handpieces was evaluated. In an initial experiment, the device partially reduced S. salivarius, and the endospores survived. In a second experiment, a 5 to 6 log reduction of S. salivarius in air and water channels was obtained. No growth was observed in clinically used high-speed handpieces, and both S. salivarius and endospores were eliminated from the turbine chambers. Thus, the method of validation proved capable of discriminating between different levels of bacterial reduction.
牙科高速涡轮机和机头在运行过程中会吸入和排出微生物，因此需要定期进行消毒。本研究建立了一种用于验证高速涡轮机和机头消毒设备的方法。空气和水通道以及涡轮腔被唾液链球菌悬浮液或嗜热脂肪芽孢杆菌的芽孢污染。通过计算从这些设备中回收的活菌数量，评估了一种结合冲洗和高压灭菌两种程序的新设备进行冲洗和/或高压灭菌的效果。此外，还评估了对临床使用的机头的影响。在最初的实验中，该设备部分减少了唾液链球菌数量，芽孢存活下来。在第二个实验中，空气和水通道中的唾液链球菌数量减少了5至6个对数级。在临床使用的高速机头中未观察到生长，并且涡轮腔中的唾液链球菌和芽孢均被消除。因此，验证方法能够区分不同程度的细菌减少情况。
