Cervoni L, Turano C, Ferraro A, Ciavatta P, Marmocchi F, Eufemi M
Department of Biochemical Sciences, A. Rossi Fanelli and CNR Center of Molecular Biology, University La Sapienza, Rome, Italy.
FEBS Lett. 1997 Nov 10;417(2):227-30. doi: 10.1016/s0014-5793(97)01285-4.
RNA polymerase II from wheat germ was analyzed for the presence of sugars. The two largest subunits and the 27 and 25 kDa subunits were found to be glycosylated by a variety of sugars. However, no N-acetylglucosamine was detected, which was found by Kelly et al. (J. Biol. Chem. (1993) 268, 10416-10424) in the largest subunit of RNA polymerase II from calf thymus. Thus it appears that the regulatory function of this sugar, postulated by Kelly et al., is performed in the wheat germ enzyme by other monosaccharides. Carbohydrate analysis of the two largest subunits of the calf thymus enzyme also revealed the presence, beside N-acetylglucosamine, of other sugars. Some similarities in the features of glycosylation of the two polymerases, isolated from very different organisms, suggest that the sugar moieties have an important role in the structure and/or function of these enzymes.
对来自小麦胚芽的RNA聚合酶II进行了糖的存在情况分析。发现两个最大的亚基以及27 kDa和25 kDa的亚基被多种糖糖基化。然而，未检测到N - 乙酰葡糖胺，而凯利等人（《生物化学杂志》（1993年）268卷，10416 - 10424页）在小牛胸腺的RNA聚合酶II最大亚基中发现了该物质。因此，凯利等人推测的这种糖的调节功能，在小麦胚芽酶中似乎由其他单糖来执行。对小牛胸腺酶的两个最大亚基的碳水化合物分析还揭示，除了N - 乙酰葡糖胺外，还存在其他糖类。从非常不同的生物体中分离出的这两种聚合酶在糖基化特征上的一些相似性表明，糖部分在这些酶的结构和/或功能中具有重要作用。
