Fleisher T A, Tomar R H
JAMA. 1997 Dec 10;278(22):1823-34.
Assays performed in the diagnostic immunology laboratory support the diagnosis and management of a wide spectrum of clinical conditions. This chapter reviews immunologic principles as they apply to diagnostic laboratory assays. Most of the determinations are based on well-established principles of antigen-antibody reactions. Some of the specific areas discussed include flow cytometric analyses, critical in the care of patients with hematologic malignancies, with the acquired immunodeficiency syndrome, or undergoing transplantation, and protein electrophoretic assays to identify the presence of monoclonal gammopathies. We also discuss the use of molecular techniques in the diagnosis of hematologic malignancies and primary immunodeficiencies, characterization of the major histocompatibility complex, and enumeration of viral burden.
诊断免疫学实验室所进行的检测有助于多种临床病症的诊断与管理。本章回顾适用于诊断实验室检测的免疫学原理。大多数检测基于成熟的抗原 - 抗体反应原理。所讨论的一些具体领域包括流式细胞术分析，这对于血液系统恶性肿瘤患者、获得性免疫缺陷综合征患者或接受移植患者的护理至关重要，以及用于鉴定单克隆丙种球蛋白病的蛋白质电泳检测。我们还讨论了分子技术在血液系统恶性肿瘤和原发性免疫缺陷诊断中的应用、主要组织相容性复合体的特征分析以及病毒载量的计数。
