Sonoda M, Harwood F L, Wada Y, Moriya H, Amiel D
Department of Orthopaedics, University of California San Diego, La Jolla 92093-0630, USA.
Am J Sports Med. 1997 Nov-Dec;25(6):755-62. doi: 10.1177/036354659702500606.
The effect of hyaluronan (molecular weight = 8 x 10(5)) on the meniscus and on the articular cartilage was assessed after partial meniscectomy in a rabbit model. On gross examination, remodeled meniscus appeared as newly synthesized translucent tissue, and was seen in both vehicle- and hyaluronan-treated menisci. Histologically, safranin O staining revealed the strong presence of glycosaminoglycans in the newly remodeled tissue, and polarized light demonstrated the absence of mature collagen architecture. Hydration of the hyaluronan-treated menisci was significantly less than that of the vehicle-treated menisci, and the reducible collagen cross-link dihydroxylysinonorleucine was significantly increased in the hyaluronan-treated menisci compared with the vehicle-treated menisci, indicative of a greater degree of collagen remodeling. In situ hybridization of vehicle- and hyaluronan-treated menisci revealed a high level of type I procollagen mRNA expression and minor expressions of types II and III mRNA. Expression of the type I collagen gene appeared to be more pronounced in the hyaluronan-treated menisci than in the vehicle-treated menisci. The tibial plateaus revealed mild cartilage fibrillation after partial meniscectomy. A statistically significant difference between vehicle- and hyaluronan-treated cartilage was not demonstrated in the present study because of the slow development (i.e., 12 weeks) of osteoarthritis after partial meniscectomy in the rabbit model. These results suggest that in the rabbit model, hyaluronan enhances collagen remodeling and inhibits meniscal swelling after partial meniscectomy in the avascular region.
在兔模型中，部分半月板切除术后评估了透明质酸（分子量 = 8×10⁵）对半月板和关节软骨的影响。大体检查时，重塑的半月板呈现为新合成的半透明组织，在载体处理组和透明质酸处理组的半月板中均可见。组织学上，番红O染色显示新重塑组织中存在大量糖胺聚糖，偏光显微镜显示无成熟的胶原结构。与载体处理组的半月板相比，透明质酸处理组的半月板水合作用明显降低，且透明质酸处理组的可还原胶原交联二羟基赖氨酸正亮氨酸显著增加，表明胶原重塑程度更高。载体处理组和透明质酸处理组半月板的原位杂交显示I型前胶原mRNA表达水平较高，II型和III型mRNA表达较少。I型胶原基因的表达在透明质酸处理组的半月板中似乎比载体处理组的半月板中更明显。部分半月板切除术后，胫骨平台显示轻度软骨纤维化。由于兔模型中部分半月板切除术后骨关节炎发展缓慢（即12周），本研究未证明载体处理组和透明质酸处理组软骨之间存在统计学显著差异。这些结果表明，在兔模型中，透明质酸可增强胶原重塑并抑制无血管区域部分半月板切除术后的半月板肿胀。
