Karawya M S, Sharaf A A, Diab A M
J Assoc Off Anal Chem. 1976 Jul;59(4):795-8.
Two colorimetric methods are presented for determining reserpine. In the first method, an iron hydroxamate complex is formed through the ester group in position 16 in the reserpine molecule. The color is measured at 535 nm (0.5-6 mg/25 ml). This method is useful for routine and control analyses of reserpine formulations. In the second method the tertiary amino group of reserpine reacts with 2% citric acid in acetic anhydride to form a red-violet complex which is measured at 505 nm (5-400 mug/10 ml). This method could be useful in measuring trace amounts of reserpine present in biological fluids.
介绍了两种测定利血平的比色法。第一种方法中，利血平分子中16位的酯基形成一种异羟肟酸铁络合物。在535nm处测定颜色（0.5 - 6mg/25ml）。该方法适用于利血平制剂的常规分析和对照分析。第二种方法中，利血平的叔氨基与乙酸酐中的2%柠檬酸反应形成红紫色络合物，在505nm处测定（5 - 400μg/10ml）。该方法可用于测定生物流体中存在的痕量利血平。
