Li Q X, Alcantara-Licudine J P, Li L P
Department of Environmental Biochemistry, University of Hawaii, Honolulu 96822, USA.
J Chromatogr Sci. 1997 Dec;35(12):573-7. doi: 10.1093/chromsci/35.12.573.
Phloxine B and uranine are color additives in drugs and cosmetics as well as potential photoactive insecticides. A capillary zone electrophoretic (CZE) method is developed to determine phloxine B and uranine in water. A fused-silica capillary (67 cm, 75-micron i.d.) and borate buffer are used. Migration of phloxine B and uranine increases slightly as the pH of the running buffer increases between the range of 8-9. Although there are only slight effects of ionic strength on the analyte migration in the range of 0-20 mM NaCl in the running buffer, the migration of phloxine B and uranine increases as the percentage of methanol in the samples increases. Methanol shows little effect on the quantitation of phloxine B and uranine. The CZE procedure is applied to determine phloxine B and uranine fortified in tap and stream water samples. Solid-phase extraction is employed to recover the analytes spiked in the water samples. Recoveries range from 87-112% for phloxine B spiked at 10-200 ppb in the tap and stream water. Uranine recoveries are 86-91% at fortification levels of 10-50 ppb in the water samples.
焰红B和荧光素钠是药品和化妆品中的色素添加剂，也是潜在的光活性杀虫剂。开发了一种毛细管区带电泳（CZE）方法来测定水中的焰红B和荧光素钠。使用熔融石英毛细管（67 cm，内径75微米）和硼酸盐缓冲液。随着运行缓冲液的pH值在8 - 9范围内增加，焰红B和荧光素钠的迁移率略有增加。尽管在运行缓冲液中0 - 20 mM NaCl范围内离子强度对分析物迁移的影响很小，但随着样品中甲醇百分比的增加，焰红B和荧光素钠的迁移率增加。甲醇对焰红B和荧光素钠的定量影响很小。该CZE程序用于测定自来水和溪流水样中添加的焰红B和荧光素钠。采用固相萃取法回收水样中添加的分析物。在自来水和溪流水样中添加10 - 200 ppb的焰红B时，回收率为87 - 112%。在水样中添加10 - 50 ppb的荧光素钠时，回收率为86 - 91%。
